Figure 2

Detailed anisotropy temperature profile. Anisotropy was measured at 585 nm in single degree increments from 20 to 48°C. Following the final measurement at 48°C, the sample was cooled to 25°C and anisotropy was re-assessed (triangle, dotted line). The data were normalized to the anisotropy value at 25°C (set to 1.0) and to the minimum value obtain in the profile (set to 0.0) prior to aggregation of results. Error bars represent the SEM for three vesicle samples.