Figure 4

Emission intensity spectra for refrigerated and equilibrated vesicles. Emission spectra were collected at 25 (thin solid curve), 30 (dotted), 35 (dashed), and 45°C (heavy solid). Panel A: Vesicles were refrigerated for at least 24 h prior to fluorescence measurements. The inset displays the details of the 25 and 30°C spectra. Panel B: Vesicles were equilibrated at 50°C prior to adjusting to the experimental temperatures used in Panel A.