Figure 5

Detailed emission peak ratio temperature profile. Emission intensities (at 585 and 621 nm) were collected in single degree increments from 20 to 50°C for refrigerated vesicles (solid circles), and the ratio of these intensities (585 nm/621 nm) was calculated. Error bars represent the SEM for four vesicle samples. The open circles denote a sample that was equilibrated at 50°C prior to cooling the sample to 25°C and then assaying the emission intensity at single degree intervals back up to 50°C. Data were normalized and scaled to the maximum ratio (set to 1.0) and the ratio of refrigerated samples at 25°C (set to 0.0).